8 Major Enzymes Involved in DNA Replication | Enzymes in DNA Replication

In this article, we will discuss about:- Major enzymes involved in the process of DNA replication. The enzymes are: 1. Helicase 2. Topoisomerase 3. Single Stranded Binding Proteins 4. Primase  5. RNA Primer 6. DNA Polymerase III  7. DNA Polymerase I  8. DNA Ligase

1. Helicase:-

The enzyme that is responsible for the unwinding of the double helix by disrupting the Hydrogen Bonds between the anti-parallel strands (bonds that bind the nitrogenous bases).

2. Topoisomerase:-

An enzyme that relieves any form of tension that's produce after the DNA double hole is unwound.

3. Single Stranded Binding Proteins:-

The proteins that bind to the outside of the two single template strands and prevent the separated strands of DNA from re-attaching.

4. Primase:-

An enzyme that builds RNA Primers and assembles them at the origin of replication site

5. RNA Primer:-

RNA primer composed of multiple bases that attached (through Hydrogen Bonds) to the template strand to initiate the DNA Replication (initiates a new complementary strand to be built)

6. DNA Polymerase III:-

An enzyme that creates complementary strands of DNA during Replication. Attaches to an RNA Primer Can only add nucleotide to a Wend of a DNA strand DNA

7. DNA Polymerase I:-

The enzyme that removes RNA primers and replaces them with the appropriate DNA nucleotide

8. DNA Ligase:-

The enzyme that joins DNA fragments together by phosphodiesterase bonds